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DXR3 Microscope

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DXR3 Microscope

NEW IN 2020: The Nicolet DXR3 Dispersive Raman Microscope is an instrument designed for applications requiring high spatial resolution, ease of sample preparation and the use of the strongpoints of Raman microscopy

  • About the spectrometer
  • Applications
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  • Accessory

The Nicolet DXR3 DispersiveRaman Microscope is an instrument designed for applications requiring high spatial resolution, simplicity of sample preparation, and the use of the strongpoints of Raman microscopy. It has been developed especially for analysts, technicians and scientists who know exactly what information they want, but do not have the time or desire to become a Raman spectroscopy specialist, and also for experts looking for better answers in a faster way. The main advantages of this unique device are:

  • Autoexposure and autofocus - like digital cameras. No more searching for optimal parameters and measuring with the "trial and error" method!
  • 3D visualization software, Particle Analysis functions for microplastic analysis, etc.
  • Spatial resolution: 540 nm in the X and Y axes, depth resolution approx. 1.7 micrometers (Z axis).
  • More than five different excitation lasers for optimal acquisition of a spectrum of difficult samples.
  • Laser power regulator for constant laser power delivered on the sample.
  • Confocal design, excellent visual quality. Reflective and transmission exposure of the sample, fluorescent illumination.
  • Patented quick automatic adjustment system for maximum performance.
  • Fast, automatic multipoint calibration for confidence in sample identification: DynaCal automatic X-axis calibration.
  • Compatibility with many high-quality Olympus microscopic parts.
  • Class 1 laser safety - no workplace modifications required.
  • Complete package of DQ/IQ/OQ/PQ validation protocols + CFR 21 part 11 compliance.
  • Možnost automatické polarizace

DXR3 Raman Microscope: The new dispersive Raman microscope

The Nicolet DXR3 Raman microscope has a unique spectrograph design, which, unlike conventional Raman spectrometers, does not require additional adjustment of the instrument when changing the excitation laser. All optional components - lasers, gratings and filters - use SmartLock technology for fully reproducible placement and automatic recognition of the connected components. The device can therefore be upgraded at any time to a higher resolution, different excitation laser wavelength, fiber optics, all without the need for additional adjustment by a service technician. It is only necessary to buy the required part (e.g. a new excitation laser) and you can measure immediately!

The microscope part of the instrument uses infinity-corrected confocal optics. Lenses and other optical parts are manufactured by Olympus. The microscope is equipped with a trinocular for visual preview and video image at the same time. The microscope head can carry up to 5 objectives - 4x, 10x, 20x, 50x, 100x, oil-immersion, water immersion, objectives with a long working distance, etc.

The illumination can be standard or brightfield/darkfield type, samples can also be illuminated by transmission. The image can be further modified by a polarization analyzer, such as a fluorescence or DIC system. The microscope stage can be manual or motorized, with two different degrees of displacement precision. The sample compartment can also hold large samples, heated, cooled and other special adapters, well-plates, etc. (according to application).

The spectrum measurement system includes autofocusto obtain the maximum intensity of the Raman signal and autoexposureto calculate the optimal exposure time and number of exposures. The spectra of fluorescent samples can be corrected automatically. The software also automatically detects and removes spectrum artifacts caused by cosmic ray particles.

To achieve maximum quality, reproducibility and wavelength accuracy of the measured Raman spectra, the Nicolet DXR3 Raman microscope is equipped with a patented tool for automatic optics adjustment, which achieves precise alignment of the laser beam, Raman emission, visible image and hair cross of the microscope. At the same time, the tool is used to calibrate the wavelengths on the emission lines of a neon lamp, to calibrate the laser frequency on polystyrene standard bands and to calibrate the intensity of the spectrum on a standardized white light source.

The device is controlled by a very user-friendly and time-tested Omnicsoftware. The identification of unknown samples is facilitated by the most extensive supplied electronic library of Raman spectra on the market. Omnic also provides the possibility of simultaneous comparison of the infrared spectrum of the sample with the database of IR spectra and Raman spectra of the sample with the database of Raman spectra (Linked Search).

Other programs, perfectly integrated with Omnic, enable automated measurement and analysis of measured spectra from well plates, etc. - Omnic Array Automation), sample mapping and image analysis (Omnic Atlµs), measurement and evaluation of time-varying samples (chemical kinetics, polymerization, curing, .. - Omnic Series), automation of routine measurements (Omnic Macros/Pro and Visual Basic), as well as quantitative and qualitative analysis of samples (TQ Analyst).

The FT-IR spectrometers iS50 can be easily expanded with all the possibilities of Raman spectroscopy, but also microscopy using the new internal FT-Raman module. It is equipped with an excitation laser 1064 nm with software selectable power.

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